Journal: bioRxiv
Article Title: Concerted remodelling of the postsynaptic spine and RNA granule by cLTP
doi: 10.1101/2025.07.16.665171
Figure Lengend Snippet: a , Experimental design of biotinylation assays in cortical neurons under basal, cLTP and post-cLTP conditions. b , A representative αFLAG immunofluorescence image of cortical neurons expressing FLAG-APEX2 fused to a nuclear export signal sequence from a lentiviral vector. c , Proteomic analysis of streptavidin pulldown and input samples from cortical neurons subject to biotin labelling under basal conditions. d , Pulldown to input ratios as a function of the predicted number of surface tyrosines per protein. e , Volcano plot with enrichment scores for the indicated selection of protein sets (bubble size indicates protein number in each protein set). f , Tyrosine surface exposure in ribosomal proteins with low and high accessibility scores as determined by their streptavidin pulldown / input ratios. g , Accessibility scores corresponding to ribosomal proteins as a function of the number of surface tyrosines hidden in the 80S ribosome under basal (blue) and cLTP (orange) conditions. h , Proteomic analysis of cortical neurons under basal and cLTP conditions. Ribosomal proteins are indicated (red dots).
Article Snippet: HEK293T cells were transfected with pFUW lentiviral vectors, envelope plasmid pVSV-G (Addgene, 8454) and packaging plasmid pCMV-dR8.2 dvpr (Addgene 8455).
Techniques: Immunofluorescence, Expressing, Sequencing, Plasmid Preparation, Selection